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ผลงานตีพิมพ์ในวารสารวิชาการOptimization of one-step real-time reverse transcription-polymerase chain reaction assays for norovirus detection and molecular epidemiology of noroviruses in thailandผู้แต่ง: Neesanant, P.,  Dr.Theerapol Sirinarumitr, Associate Professor , Dr.Sirirak Chantakru, Assistant Professor, Dr.Ukadej Boonyaprakob, Assistant Professor , Chuwongkomon, K., Bodhidatta, L., Sethabutr, O., Abente, E.J., Supawat, K., Mason, C.J., วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการสภาวะที่เหมาะสมในการทำปฏิกิริยาลูกโซ่โพลีเมอเรสแบบย้อนกลับเพื่อตรวจหาเชื้อเคไนน์โคโรนาไวรัส (2017)ผู้แต่ง:Puthiphak Mutthakalin, Thanachote Tangporntawee, Jurairat Thiratantikul, Thepsopa Assawateerakiat, Ms.natnaree inthong, Assistant Professor , Dr.Sarawan kaewmongkol, Associate Professor , Dr.WUTTINUN RAKSAJIT, Associate Professor , วารสาร: |
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ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 47หัวเรื่อง:การพัฒนาปฏิกิริยา TaqMan real-time reverse transcription-polymerase chain reaction เพื่อการตรวจหาปริมาณเชื้อ Feline Leukemia Virus |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of TaqMan? Real-time Reverse Transcription-Polymerase Chain Reaction for the Quantification of Feline Leukemia Virus Load) ผู้เขียน:วิชญะ ทองตะโก, Therapol Sirinarumitr สื่อสิ่งพิมพ์:pdf AbstractFeline leukemia virus (FeLV), a gamma retrovirus of the domestic cat, is not only of veterinary interest, but is also an important model for the study of pathogenesis of tumors and AIDS in many animals. After initial infection, disease progression has been associated with cellular and humoral immune response and FeLV proviral load. Moreover, an earlier study showed that not only the proviral load, but also the plasma viral loads are important parameters, which are associated with progression of the disease. In this study, the TaqMan? real-time reverse transcription-polymerase chain reaction (RT-PCR) was developed for the quantification of FeLV loads. The assay was developed to amplify a 131 base pairs conserved domain within the unique region (U3) of a long terminal repeat (LTR). The detection limit of this assay was 8.3 copies of RNA standard template or 4.15 viruses per reaction that is equivalent to 1,778 viruses per ml of plasma or serum. |
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ผลงานตีพิมพ์ในวารสารวิชาการHigh-resolution melting analysis for simultaneous detection and discrimination between wild-type and vaccine strains of feline calicivirusผู้แต่ง:Phongroop, K., Dr.Jatuporn Rattanasrisomporn (Noosud), Associate Professor, Tangtrongsup, S., Rungsipipat, A., Piewbang, C., Techangamsuwan, S., วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการDevelopment of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farmsผู้แต่ง:Meachasompop, P., Mr.Anurak Uchuwittayakul, Lecturer, Keaswejjareansuk, W., Dechbumroong, P., Namdee, K., Dr.Prapansak Srisapoome, Associate Professor , วารสาร: |
ที่มา:Thai Journal of Veterinary Medicineหัวเรื่อง:การวัดระดับการแสดงออกของ miRNA-29a ในเซลล์เม็ดเลือดขาวสุกรด้วยเทคนิคพีซีอาร์เชิงปริมาณ |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Polyclonal Antibodies Specifi c to the Recombinant Coat Protein of Blackeye cowpea mosaic virus and Its Use in Disease Detection) ผู้เขียน:Maneerat Koohapitagtam, Charassri Nualsri สื่อสิ่งพิมพ์:pdf AbstractThe coat protein gene of Blackeye cowpea mosaic virus (BICMV-CP) was amplifi ed by reverse transcription polymerase chain reaction and cloned into the expression vector pQE-80L. This plasmid was transformed into Escherichia coli DH5? competent cells. The BICMV-CP gene was expressed as a fusion protein containing a fragment of 6xHis-tag. Bacterial cells were disrupted by repeated freezethawing three times and the BICMV-CP fusion proteins were purifi ed under denaturing conditions by affi nity chromatography with Ni-NTA Agarose. A sample of 500 ?g of purifi ed protein was mixed with Freund’s complete adjuvant at a ratio of 1:1 (volume to volume). Initially, the emulsion was subcutaneously injected into a New Zealand White rabbit, followed at weekly intervals by three additional immunizations with 500 ?g of the purifi ed protein mixed with Freund’s incomplete adjuvant. Bleeding was done every week during weeks 5-12 and titers of the antisera ranging from 800-51,200 were obtained. Up to a dilution of 1:320 of the BICMV-infected yardlong bean sap could be detected by indirect enzyme-linked immunosorbent assay. The produced antiserum reacted specifi cally with the BICMV-infected plant without any cross reaction with other virus species tested. However, a weakly positive reaction with Bean common mosaic virus could be observed. |
ผลงานตีพิมพ์ในวารสารวิชาการHuman BST2 inhibits rabies virus release independently of cysteine-linked dimerization and asparagine-linked glycosylationผู้แต่ง:Tanwattana, N., Wanasen, N., Jantraphakorn, Y., Srisutthisamphan, K., Chailungkarn, T., Boonrungsiman, S., Lumlertdacha, B., Dr.Porntippa Lekcharoensuk, Professor , Kaewborisuth, C., วารสาร: |